Online Determination of the Enantiomer Concentration Using HPLC Coupled with UV and Circular Dichroism Detectors

The application of ultraviolet absorption detector (UV) and circular dichroism (CD) detectors in series in HPLC systems has been increased in recent years. In this work, an effective method to determine the enantiomer concentration on line in chiral drugs was explored. Ibuprofen (IBP) racemate was applied. The total concentration and concentration difference of S-(+)-IBP and R-(-)-IBP were obtained by the online monitoring systems in HPLC systems. The single enantiomer respective concentrations were also calculated. The UV and CD detectors in series as the online monitoring systems to determine the enantiomer concentration was applied. This method is gradually developed into a practical tool for enantiomeric purity analysis. A new method which can fit the two components respectively rather than both components in the fitting procedure of Inverse method (IM) was provided. This research shows that a commercially available UV and CD detectors in series can be successfully used to determine the enantiomer concentration.